northern blot analysis

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    • #11634
      proteinz
      Participant

      hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help…
      i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
      thanks in advance
      😀

    • #92314
      MrMistery
      Participant

      maybe unprocessed and processed RNA?
      I have never used northern blots (honestly I didn’t know anyone still used them) so I’m just taking a guess here

    • #92623
      robsabba
      Participant
      quote proteinz:

      hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help…
      i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
      thanks in advance
      😀

      Is it possible you are picking up a mRNA from a similar gene that produces a larger transcript?

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