In our course ‘analytical molecular biology’ (last master year – University of Ghent) some techniques are described. Amongst them the nuclear run-on assay. In the course the professor gave a remark but we (the students) can’t make anything of it. Maybe some of you can?
First he described the nuclear run-on assay and then he says (i’m just copying it):
Remark: by comparing a run-on with a DNA-probe corresponding to the 5′ end, and a run-on assay with a DNA-probe corresponding to the 3′ end of the gene, one can get an idea whether there is also an involvement of regulation of elongetion.
5′ probe 2x
3′ probe 20x
conclusion: important contribution of elongation
He didn’t explain it is his lesson and the above text is all there is written. Anyone?