nucleus stained

Viewing 5 reply threads
  • Author
    Posts
    • #7996
      yangp
      Participant

      Dear all:

      I stained all the nuclei in my immunohistochemistry study. Do you know what is the reason, how do I improve it.

      Thanks

      Parker

    • #74799
      sdekivit
      Participant

      you stained the nuclei with DAPI ? well it’s much easier to find your cells if your nucleus is stained 😆

    • #74803
      yangp
      Participant

      No, just with the normal enzymatic procedures, primary antibody, HRP-conjugated antibody and DAB.

    • #74840
      sdekivit
      Participant

      to what was your primary antibody ? and hat is the main goal of the study :p otherwise it will be hard to tell the function of staining the nucleus.

    • #75880
      ab06
      Participant

      What antibody are you staining for? Maybe background staining or your primary is not specific.

    • #75881
      ab06
      Participant

      by the way. There is an nuclear antibody called "Nuclei" you may want to give it a try.

Viewing 5 reply threads
  • You must be logged in to reply to this topic.