optimum molar ratio for pET28 cloning?

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    • #14246
      patlangic
      Participant

      Hello everyone..I wonder if someone could help me find the optimum molar insert:vector ratio (or ratio range) to clone my gene into the pET28a(+) vector. If there is anyone who used this expression vector before, I would appreciate any recommendation. Thanks in advance..

    • #102669
      JackBean
      Participant

      I think you could use the regular 1:3 ratio 😉

    • #102671
      patlangic
      Participant

      OK, I’ll try it. Thank you 🙂

    • #102876
      Kezzer
      Participant

      Hey, I used PET28c using a molar ration of 1:8 if 1:3 isn’t suitable maybe try that 🙂

    • #102879
      patlangic
      Participant
      quote Kezzer:

      Hey, I used PET28c using a molar ration of 1:8 if 1:3 isn’t suitable maybe try that 🙂

      what a coincidence 🙂 I just tried 1:7 and 1:3 ratios and performed transformation, so I’ll get the results in a couple of days. Thank you..

    • #102886
      Kezzer
      Participant

      No problem, hope it works 🙂

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