Organism-pathogen challenge protocol

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      Jevandrix
      Participant

      Hi everyone,

      I am doing a transcriptomic study on fish immunogenomics to study expressed immune genes, which involves fish-pathogen challenge. To do this I would like to know the parameters that I need consider. Especially, what is the appropriate concentration of bacteria to be used? Is strain-specific or fish species specific or both.

      For additional info I am will be working on Asian seabass Lates Calcarifer challenged with live V. alginolyticus. But I do not know what concentration to use, is it dependant on LD-50, Lethal concentration or something else?

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