Orientation of genes

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    • #17214
      Sue4
      Participant

      When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have placed in one direction and the marker gene has placed in the opposite direction. Is there any reason to do so?

      Thank you very much 🙂

    • #113448
      Sue4
      Participant

      "to be cloned" part is not needed. Sorry

      When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have placed in one direction and the marker gene has placed in the opposite direction. Is there any reason to do so?

      Thank you very much

    • #113450
      JackBean
      Participant

      I’d say it’s in opposite direction to abolish transcriptional fusion.

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