PCR and Gel conundrum

Viewing 3 reply threads
  • Author
    Posts
    • #15326
      antonyviridae
      Participant

      Why is it that when I run the exact same PCR reaction (with the same sample) on two different PCR platforms, that when I run it on a gel or on Qiaxcel I see that the bands are running at different sizes? The difference isn’t significant but enough to be noticable.

    • #106554
      merv
      Participant

      I dont know.
      Agarose gels are different technology to the patented system to which you refer, so I would ask the Qiagen reps to pay you a visit and explain it. or email them. or call.
      it may be related to the pH of the gel and buffer, gel concentration, etc.

    • #106779
      JackBean
      Participant

      I understood the question that there is difference between two PCRs; not between home-made gel and Qiaxcel.

    • #108218
      tranglee
      Participant

      you could check your technique, maybe have some mistakes during your working

Viewing 3 reply threads
  • You must be logged in to reply to this topic.