PCR

[charanT]

301 5’gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg ggtcaaggtg
361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag catgtggcag
421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg ggaagtgcca
481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca 3′

-A diagram indicating where any primers or probes would anneal to
the above sequence (you can annotate the sequence above).
-A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.
-A statement of how you will detect the polymorphisms
-Controls needed
-The concentrations and formulations of any gels used
-The concentrations and components of any enzymatic reactions used
-A diagram of how the results of your assay would appear including
controls, a homozygous individual and a heterozygote.

[mith]

I hope there’s a text/lecture associated with this.

[charanT]

I am not sure what do you mean? this is the problem, mainly I can’t figure out the detecting polymorphism, homozygote and hetrozygote?

[snowcapk]

I pity the fool who is heterozygous for sheep prion protein…

[Author]

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