-A diagram indicating where any primers or probes would anneal to
the above sequence (you can annotate the sequence above).
-A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.
-A statement of how you will detect the polymorphisms
-The concentrations and formulations of any gels used
-The concentrations and components of any enzymatic reactions used
-A diagram of how the results of your assay would appear including
controls, a homozygous individual and a heterozygote.