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    • #9490
      charanT
      Participant

      301 5’gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg ggtcaaggtg
      361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag catgtggcag
      421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg ggaagtgcca
      481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca 3′

      -A diagram indicating where any primers or probes would anneal to
      the above sequence (you can annotate the sequence above).
      -A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.
      -A statement of how you will detect the polymorphisms
      -Controls needed
      -The concentrations and formulations of any gels used
      -The concentrations and components of any enzymatic reactions used
      -A diagram of how the results of your assay would appear including
      controls, a homozygous individual and a heterozygote.

    • #83518
      mith
      Participant

      I hope there’s a text/lecture associated with this.

    • #83524
      charanT
      Participant

      I am not sure what do you mean? this is the problem, mainly I can’t figure out the detecting polymorphism, homozygote and hetrozygote?

    • #83527
      snowcapk
      Participant

      I pity the fool who is heterozygous for sheep prion protein…

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