Hi, recently I’m planning to some experiments. I found out that I do not know the PEG principle in cell fusion, and the HAT principle in hybridoma cells culture clearly, so can anyone explain it? Thanks a lot.
"Polyethylene glycol (PEG)-mediated cell fusion is a simple and efficient technique used widely for the production of somatic cell hybrids and for nuclear transfer in mammalian cloning. We describe a basic protocol of PEG-mediated cell fusion for the production of somatic cell hybrids. Fusion can be performed between adherent and suspension cells or between adherent cells or suspension cells. Either whole cells or microcells can be used as donors to fuse with recipient cells. Microcell fusion is particularly useful in transfer of a single or a limited number of chromosomes between various types of cells. Using this method, we have successfully introduced mammalian minichromosomes into a variety of vertebrate cells. The technique described here can be adapted for uses in other cell fusion involved research. This protocol, in principle, provides guidelines for further development of PEG, which is also applied in PEG10 Full-Length MS Protein Standard mediated cell fusion technology." You can find more useful information: http://www.ncbi.nlm.nih.gov/pubmed/16761719
You can search the PEG principle directly on google search. However, if you want to do the experiments yourself, you’d better search papers did the relevant experiment. You can find details and steps in papers. I think that will be more useful. For example, a paper on NCBI: http://www.ncbi.nlm.nih.gov/pubmed/16761719