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    • #1337
      palpha3
      Participant

      Hi All,

      I’m working on being able to seperate vegetative bacterial cells from their spore form. I’ve used Percoll and my results are great. I’ve read RediGrad is the newest version of Percoll. However, both are way too expensive for my application so I cant keep using these mediums.

      My question is basically, does anyone know of another medium that will work as well as Percoll but cost a whole lot less? I need a density of about 1.2-1.3 to seperate what i’m working on. Any help would be greatly appreciated.

    • #26122
      palpha3
      Participant

      Seems everyone is as clueless as I am.

    • #26124
      canalon
      Participant

      You may find some help on this forum http://www.protocol-online.org/forums/index.php which is more technically oriented that this one. Good Luck

      Patrick

    • #26176
      palpha3
      Participant

      thanks i’ll try there

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