Im trying to do a FISH on DNA combing for pericentromeric regions.
As you may know, the human centromere is actually separated into two distinct regions: 1) centromeric region – which is involved in kinetochore binding 2) pericentromere region – a heterochromatic region which is involved in sister chromatid binding.
Im looking for pericentromere specific probes which do not overlap with any other region (centromeric or non centromeric).
Does anyone have a clue whether this kind of probes/BAC/HAC/anything else exist? if so – a link would be great!