Plant hormone experiment!

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    • #16457
      duherro
      Participant

      Got given a practice prac on plant hormones.

      Method:
      1. Place 8 corn seeds (with 2 each facing towards the 4 poles, so North, East, South, West).

      2. Cover seeds with a piece of filter paper

      3. Pack petri dish with moist cotton wool.

      4. Put lid on the petri dish and seal it with sticky tape.

      5. Stand petri dish upright attached on plasticine and near a window.

      6. Let it stand for 1 week and observe.

      Q1 )The question asked if the above was a controlled experiment or not.
      I think that it was not a controlled experiment as there weren’t any variables subject to any change. (Not too sure about this question). However, maybe the north and south placed seeds could be the controls to show the direction of growth of shoots?

      Q2) What differences in growth were visible over the period of the investigation? And suggest any reasons for any differences of growth and explain how it could be altered.
      The shoots all grew vertical, while the roots will grow down.
      auxin ?

      Need help on writing an aim and hypothesis!

    • #110905
      JackBean
      Participant

      so did the seeds stay oriented to the two poles (there are no poles in East and West :roll:)? Or did you change it?

    • #110909
      duherro
      Participant
      quote JackBean:

      so did the seeds stay oriented to the two poles (there are no poles in East and West :roll:)? Or did you change it?

      Like on the petri dish , imagine there are 4 points, N E S W. And in each of those directions we put 2 seeds in there. Btw, do you hhave any IM ?

    • #110910
      JackBean
      Participant

      yeah, I understand that. Does the Petri dish stay as it is or is it turned to be vertical or upside-down? What’s the aim of the experiment?

    • #110912
      duherro
      Participant
      quote JackBean:

      yeah, I understand that. Does the Petri dish stay as it is or is it turned to be vertical or upside-down? What’s the aim of the experiment?

      The petri dish is just upright and left alone for 1 week. The aim is most likely to observe the growth reponse from seeds to light and gravity ? Got skype or something?

    • #110913
      JackBean
      Participant

      And how does gravity differ depending on whether is the seed facing toward North or West?

      Sorry, I don’t have Skype or anything

    • #110914
      duherro
      Participant
      quote JackBean:

      And how does gravity differ depending on whether is the seed facing toward North or West?

      Sorry, I don’t have Skype or anything

      Well just imagine it will be like

      >N
      W E
      >S

      and in each of the poles, there are 2 seeds in them

    • #110916
      JackBean
      Participant

      Is it vertical or horizontal?

    • #110918
      duherro
      Participant
      quote JackBean:

      Is it vertical or horizontal?

      Its facing upright, so vertical

      like standing up , your head is the N, your arms are E and W and your feet are S. sorta like that

    • #110919
      JackBean
      Participant

      OK, so the West, East, North and South are only for confusion…

      Yep, in such case you’re researching the effect of gravity. And yes, auxin flux is responsible for that.

    • #110920
      duherro
      Participant
      quote JackBean:

      OK, so the West, East, North and South are only for confusion…

      Yep, in such case you’re researching the effect of gravity. And yes, auxin flux is responsible for that.

      could you suggest me a possible hypothesis?

      And also…

      Suggest a biomolecular reason for any diffrences observed in the growth of plant and explain how the growth was alterted.

    • #110921
      JackBean
      Participant

      Hypothesis why or how?

      The biomolecular reason here was the auxin and PIN proteins 🙂

    • #110923
      duherro
      Participant
      quote JackBean:

      Hypothesis why or how?

      The biomolecular reason here was the auxin and PIN proteins 🙂

      Damn, idk about PIN proteins yet , but could you explain auxin and how it effects it? and maybe phototropism and geotropism?

    • #110924
      JackBean
      Participant

      PINs are transporters of auxin which are localised on one side of cell forming polar auxin transport. And their localisation is driven by several factors…

    • #110925
      duherro
      Participant
      quote JackBean:

      PINs are transporters of auxin which are localised on one side of cell forming polar auxin transport. And their localisation is driven by several factors…

      hmm what happens if auxin promotes apical dominance, then it cant grow anymore due to the limiting space?

    • #110926
      JackBean
      Participant

      The apical bud is growing.

    • #110927
      duherro
      Participant
      quote JackBean:

      The apical bud is growing.

      So lateral growth wont start, the apical bud will probaby just bend due to the circular shape of the petri dish?

      Also what other aspects of the plant growth and development that are regulated by auxin ? In your response, you MUST include how its action would differ for different cell tpyes/ environmental stimuli? 😀

      TY TY

      Btw , when a plant responds to an environmental stimulus, what is this action called? A: Tropism.
      And what are the 2 ways in which the plant respond to a stimulus: Negative and positive.

    • #110929
      JackBean
      Participant

      I don’t have to, but you should

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