Plasimd prep from frozen cells

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    • March 29, 2013 at 1:04 am #17301
      biology_06er
      Participant

      Hi there,

      I read in a protocol that that it is possible to extract plasmid from frozen cells using a kit? How would one do use frozen cells? Would you take a glycerol stock, thaw it out, spin it and use pellet?

    • March 29, 2013 at 2:07 pm #113693
      JackBean
      Participant

      Just grow the cells, spin them down, freeze and keep at -80°C until used.

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