Plasmid mini prep

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    • #13226
      testsubjectzz
      Participant

      So I did a plasmid mini prep and you forgot to use RNAse would it be suitable for cloning, i’m guessing not but hoping yes.

      I will be running a gel tomorrow to check it and i’m pretty sure i’ll need to repeat it 😕

      Right now it’s in -20 dissolved in distilled water or something could I possibly centrifuge that take out the water and re dissolve it in a buffer with RNAse?

      Thanks

    • #99446
      JackBean
      Participant

      I have never used RNase when isolating DNA and it was always fine. The DNA is more stable than RNA and there are everywhere plenty of RNases, so I wouldn’t worry…

    • #99450
      Jillo725
      Participant

      I wouldn’t change the protocol. but what you could do is google protocol for mini preps and see if anyone else made the same mistake and has an answer for you.

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