So I did a plasmid mini prep and you forgot to use RNAse would it be suitable for cloning, i’m guessing not but hoping yes.
I will be running a gel tomorrow to check it and i’m pretty sure i’ll need to repeat it 😕
Right now it’s in -20 dissolved in distilled water or something could I possibly centrifuge that take out the water and re dissolve it in a buffer with RNAse?
I have never used RNase when isolating DNA and it was always fine. The DNA is more stable than RNA and there are everywhere plenty of RNases, so I wouldn’t worry…
I wouldn’t change the protocol. but what you could do is google protocol for mini preps and see if anyone else made the same mistake and has an answer for you.