Poly A >>> what the propose ?

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    • October 31, 2010 at 7:52 am #14044
      Nmr
      Participant

      Hi guys i need ur help >>>

      what the propose of adding the poly A tail to the 5′ terminus of the primer in PCR in Megaprimer method ?

      thanx

    • October 31, 2010 at 5:00 pm #102080
      JackBean
      Participant

      do you have somewhere some protocol or principle? I was looking on Google, but there are only original articles…

    • November 1, 2010 at 4:02 am #102089
      Nmr
      Participant

      Actually.. i do by Megaprimer… using certain protocol… and as i know it’s better to add poly A tail, but the problem is my lecturer said that no need to add poly A tail.?! so now i got confuse about the function of Poly A tail and what the propose of adding it ??!!

      pleas if anyone know about that, can he/she give a brief explanation about the poly A function?

      thanx

    • November 1, 2010 at 7:42 am #102091
      JackBean
      Participant

      I thought, whether could you provide us with some protocol?

    • November 1, 2010 at 9:08 am #102098
      Nmr
      Participant

      http://www.protocol-online.org/prot/

      here u can get whatever protocol u want…

    • November 1, 2010 at 10:00 am #102100
      JackBean
      Participant

      did not find

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