Biology Forum › Molecular Biology › Poly A >>> what the propose ?
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AuthorPosts
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October 31, 2010 at 7:52 am #14044
Nmr
ParticipantHi guys i need ur help >>>
what the propose of adding the poly A tail to the 5′ terminus of the primer in PCR in Megaprimer method ?
thanx
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October 31, 2010 at 5:00 pm #102080
JackBean
Participantdo you have somewhere some protocol or principle? I was looking on Google, but there are only original articles…
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November 1, 2010 at 4:02 am #102089
Nmr
ParticipantActually.. i do by Megaprimer… using certain protocol… and as i know it’s better to add poly A tail, but the problem is my lecturer said that no need to add poly A tail.?! so now i got confuse about the function of Poly A tail and what the propose of adding it ??!!
pleas if anyone know about that, can he/she give a brief explanation about the poly A function?
thanx
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November 1, 2010 at 7:42 am #102091
JackBean
ParticipantI thought, whether could you provide us with some protocol?
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November 1, 2010 at 9:08 am #102098
Nmr
Participanthttp://www.protocol-online.org/prot/
here u can get whatever protocol u want…
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November 1, 2010 at 10:00 am #102100
JackBean
Participantdid not find
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