Poly A >>> what the propose ?

[Nmr]

Hi guys i need ur help >>>

what the propose of adding the poly A tail to the 5′ terminus of the primer in PCR in Megaprimer method ?

thanx

[JackBean]

do you have somewhere some protocol or principle? I was looking on Google, but there are only original articles…

[Nmr]

Actually.. i do by Megaprimer… using certain protocol… and as i know it’s better to add poly A tail, but the problem is my lecturer said that no need to add poly A tail.?! so now i got confuse about the function of Poly A tail and what the propose of adding it ??!!

pleas if anyone know about that, can he/she give a brief explanation about the poly A function?

thanx

[JackBean]

I thought, whether could you provide us with some protocol?

[Nmr]

http://www.protocol-online.org/prot/

here u can get whatever protocol u want…

[JackBean]

did not find

[Author]

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