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    • #13712
      pmngrad
      Participant

      When a poly(a) signal is placed downstream of a gene sequence wouldn’t this cause the termination of transcription before the gene of interest is actually expressed?

    • #101103
      JackBean
      Participant

      how long is the poly(A) signal? Are both genes in frame?

    • #101110
      pmngrad
      Participant

      Both genes would be in frame – unsure about the exact Poly(a) signal though. I would assume that it would be a AAUAAA or equivalent sequence.

      Thanks for your help! 🙂

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