Porin-deficient mutant

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    • #16324
      Layd33foxx
      Participant

      ❓ Some bacteria become resistant to tetracycline because they don’t make porins. Why can a porin-deficient mutant be detected by its inability to grow on a medium containing a single carbon source such as succinic acid?

    • #110533
      AstraSequi
      Participant

      What is the function of a porin?

      Knowing that, what sort of experiment might you design to see whether or not the porin is present? A system where the cell dies if the porin is not present (that is, the cell can’t execute the porin’s function) is usually very convenient.

    • #110541
      Layd33foxx
      Participant

      Porins are channels control the transport of substances in and out of the cell, imparting the membrane its property of semipermeabililty.

    • #110542
      Layd33foxx
      Participant

      Bacteria can develop resistance to the antibiotic by mutating the gene that encodes the porin – the antibiotic is then excluded from passing through the outer membrane

    • #110578
      AstraSequi
      Participant

      Now suppose that the porin actually transports more than one substance – say, it can act on succinic acid as well as tetracycline. How might you figure out whether the porin is working or not? 🙂

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