Precipitation of BSA

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    • #12941
      dhkwak
      Participant

      Hey guys,

      I have precipitated BSA using by:

      (i) adding cold, dry acetone in 5 or more quantities per protein solution,
      (ii) incubating at -20 Celsius for at least an hour, and
      (iii) centrifuging at 13,000 g for 15 min and removing the supernatant.

      I wanted to know if anyone has had issues with using the acetone precipitation method for BSA. I had read that BSA can be effectively precipitated using ammonium sulfate (I am uncertain of the concentrations but have read usually 75% will suffice). Does the acetone method result in aggregation? Any help would be greatly appreciated! Thanks

    • #100834
      JackBean
      Participant

      I think it shouldn’t, if you add it slowly. But that’s why I like more using ammonium sulphate instead of organics…

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