(i) adding cold, dry acetone in 5 or more quantities per protein solution,
(ii) incubating at -20 Celsius for at least an hour, and
(iii) centrifuging at 13,000 g for 15 min and removing the supernatant.
I wanted to know if anyone has had issues with using the acetone precipitation method for BSA. I had read that BSA can be effectively precipitated using ammonium sulfate (I am uncertain of the concentrations but have read usually 75% will suffice). Does the acetone method result in aggregation? Any help would be greatly appreciated! Thanks