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    • #12745
      Crystal25
      Participant

      Hi guys, just wondering if anyone has ever made a lysis buffer also called "Relax Buffer"? If so can you let me know how much for 200ml? Many thanks

    • #97483
      JackBean
      Participant

      there apparently isn’t only one relax buffer as one link says:
      10 mM EGTA, 25 μM CaCl2, 20 mM MOPS, 50 mM KCl, 6.8 mM MgCl2, 12 mM phosphocreatine, 5 mM Na2ATP, …
      http://www.sciencedirect.com/science?_o … 0a7eb1fe3b
      and the other:
      10 mM HEPES, pH 7.4, 100 mM KCl, 10 mM NaCl, 1mM EDTA, 1 mM PMSF, 10 ug/ml chymostatin
      http://www.jleukbio.org/cgi/reprint/54/6/572.pdf

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