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    • #10469
      Dec
      Participant

      Hi all,

      For a project I’m currently working on I need to transfect a disease cell line with DNASE 1 to investigate its interaction. The problem is this molecule has a role in cell apoptosis. Is there a way I can transfect my cell lines without nuclear DNA destruction?

      Many thanks in advance for any advice offered.

    • #87239
      blcr11
      Participant

      Put the gene under the control of an inducible promoter. That way your introduced DNAse can be selectively expressed.

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