I’m in a new lab where they use short (30 nt), desalted primers to do the QuikChange rxn. Stratagene recommmends HPLC or PAGE purified primers. Using ordinary primers, I get a nice yield of PCR product, but very few transformants. I used to use PAGE purified primers and got more transformants. The transformation efficiency of the cells is high (10 to the 10). Seems the PCR product is not propagated well. Any ideas?
Sorry if this topic has been discussed previously.