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    • #10821
      minabayardi
      Participant

      HI! ¡HOLA!

      I HAVE A QUESTION ABOUT THE TOPIC. IF SOME ONE CAN HELP ME, PLEASE, I NEED IT. I AM DESIGNING PCR OLIGOS, AND IN THE FUTURE WE COULD USE THEM TO SCREENING A DNA LIBRARY. IS TOTALLY IMPORTANT THAT THE PRIMERS ARE IN READING FRAME? IF THE ANSWER IS YES, PLEASE TELL ME HOW TO DO IT.
      THANKS.
      I AM VERY SORRY BECAUSE OF MY BAD ENGLISH, PLEASE IMAGINE THAT IS NOT BAD AT ALL. SORRY.

      MINA.

    • #88726
      canalon
      Participant

      No.

      But you also want to stop posting all in capital letters. On the internet, it is similar to shouting. And that is rude.

    • #88744
      minabayardi
      Participant

      Well, I did not know… I do not wanted be rude in any posible way. I have to apologize for do not know this rule.

      Mina

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