So I need help on how to do this graph correctly:

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The experiment is Enumeration of Bacteria:The standard plate count

My results for the Quantitative plating method is:

Dilution Bottle — ml Plated — Dilution — Dilution Factor — Number of colonies

b(1:10,000) — 1.0 — 1:100,000 — 10^4 — TMC

b(1:10,000) — 0.1 — 1:1,000,000 — 10^5 — TMC

c(1:1,000,000) — 1.0 — 1:1,000,000 — 10^6 — 267

c(1:1,000,000) — 0.1 — 1:10,000,000 — 10^7 — 31

b) how many cells per ml were in the undiluted culture?___I didn’t get this answer ðŸ˜³

Second Part:

Optical density determination

Dilution — Optical Density

1:1 — 30% T; 0.56 A

1:2 — 50% T ; 0.33 A

1:4 — 72% T; 0.16 A

1:8 — 85% T; 0.09 A

1:16 — 91% T; 0.05 A

The graph x-axis is Dilution & the y is Optical Density. What number or calculation am I suppose to do to get the optical density because if I use A in optical density my graph goes down negative I dont think it’s correct. Please help. Thank you.