In the purification of plasmid DNA (of E.coli), 6 reagents were used:
Cell resuspension solution, cell lysis solution, alkaline protease, neutralising solution, column wash solution, as well as nuclease free water.
Components of the reagents are as such:
Cell resuspension solution:
Cell lysis solution:
Protease enzyme subtilisin (produced by Bacillus licheniformis)