Question on sequencing

Viewing 3 reply threads
  • Author
    Posts
    • #6772
      aaroncjj_2020
      Participant

      i’ve got a result from my sequencing. when i blast it (eg. aattgcg), it is not related to the spesies that i wan. when i blast it reversely (eg. gcgttaa), it shows that it is related to my spesies. how can we explain it?

    • #66699
      mith
      Participant

      inversion mutation

    • #66709
      sachin
      Participant

      Or sequence may be in that manner…….? I guess…

    • #66787
      aaroncjj_2020
      Participant

      if it is a an inversion mutation, how can the primers bind the sequence correct during RT-PCR as the 5′ and 3′ is already diff? thanks

Viewing 3 reply threads
  • You must be logged in to reply to this topic.