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    • #14133
      TechAssistantFTW
      Participant

      To decontaminate my bench before doing PCR I’m following this advice here:

      http://bergman-net.com/Service_web/Remo … 0DNA_1.pdf

      It suggests using a 5-10% solution of Sodium hypochlorite so I bought a bottle of this:

      http://uk.vwr.com/app/TechnicalDataSheet?catnum=1385932

      It is labelled "Sodium hypochlorite ± 3,5 % active" and I thought it’s a 3,5% solution of NaOCl, but I guess the 3,5% means something else? What exactly and can I use it for decontamination?

    • #102300
      canalon
      Participant
      quote TechAssistantFTW:

      To decontaminate my bench before doing PCR I’m following this advice here:

      http://bergman-net.com/Service_web/Remo … 0DNA_1.pdf

      It suggests using a 5-10% solution of Sodium hypochlorite so I bought a bottle of this:

      http://uk.vwr.com/app/TechnicalDataSheet?catnum=1385932

      It is labelled “Sodium hypochlorite ± 3,5 % active” and I thought it’s a 3,5% solution of NaOCl, but I guess the 3,5% means something else? What exactly and can I use it for decontamination?

      It is labeled "Sodium hypochlorite ± 3,5 % active Cl" The active Cl is the disinfecting part of the NaOCl solution. This is what you nedd, and it appears that what you bought is slightly less concentrated that what is recommended in the protocol you linked to. But I guess you could just go to the nearest shopping mall and buy a bottle of no name thin bleach (hypochlorite based of course) should have the percentage of active chlorine somewhere on the label.

    • #102434
      JackBean
      Participant

      hypochlorite quite smells, I think it’s more convenient to use simply EtOH 😉

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