Restriction Digesion

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    • #17804
      Helics
      Participant

      which is the best option for Restriction Endonuclease digesion ???

      A) 37′ C water bath
      B) 37′ C PCR program

    • #115227
      MOmarKhan
      Participant

      Both of them are okay! But i would go for Thermo-cycler instead of water bath.

    • #115229
      Helics
      Participant

      Dear scientist, Jack Bean I need your response.

    • #115245
      JackBean
      Participant

      thanks for considering me a scientist 😀

      I don’t see much difference if you’re sure that the temperature iscorrectly set. However, with thermocycler (or thermoblock) you do not have to worry, that you will spill your samples 🙂

    • #115798
      Javedz2001
      Participant

      Water bath is NOT recommended because after time the sample will evaporate and stick on the top of the cap. This will change the buffer composition. Always use PCR machine with heated lid or dry incubator.

      Javed Siddiqi

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