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    • #16723
      siddharthsameer
      Participant

      hello I need to ask one question and I am stuck with this kind;y help me…
      The concentration of my pcr product is 33.56microgram/ml and I want to make the restriction digestion .I want to have the 2 microgram of my product in the ristriction digestion volume of 50microlitre. How much volume should i add of my pcr product to the reaction volume for the restriction…
      kindly let me know I am stuckk and can not find the answer….please please help me

    • #111997
      JackBean
      Participant

      let’s say it’s 30 ng/ul and you want 2000 ng, how many-times is it? 2000/30 ~60-times -> thus you would need 60 ul

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