ribosome stall

Viewing 3 reply threads
  • Author
    Posts
    • #1695
      taotaopei
      Participant

      If I anneal a DNA oligo to the end of a mRNA strand, then perform in vitro translation. My question is can this double helix stall ribosome or not? Thanks.

    • #28632
      sdekivit
      Participant

      depends on where you put it. If you have a mature mRNA and after it you put the DNA, nothing will happen, because the stopsignal is in front of the poly-A-tail and the 3′ UTR of the mRNA.

    • #28639
      taotaopei
      Participant

      The DNA oligo is complementary to the mRNA before the stop codon. So, what will happen. Is there any pape about this?

    • #28671
      sdekivit
      Participant
      quote taotaopei:

      The DNA oligo is complementary to the mRNA before the stop codon. So, what will happen. Is there any pape about this?

      i think that the translation will be stopped, bacause there’s an obstacle for the ribosome. Eeventually it will fall of. But this is not based on facts, just my guess.

      I don’t know if this is tried before in an experiment.

Viewing 3 reply threads
  • You must be logged in to reply to this topic.