RNA and amine groups ?

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    • #11948
      strasbarge
      Participant

      Hi !

      I would like to know if it is possible to link an amine group to a RNA. This is in order to link a fluorochrome and follow its shifting. Or does anyone have any idea if there is a kit to bind fluorochrome to RNA ?

      Thanks per adavance for your help !!

      Strasbarge

    • #93381
      JackBean
      Participant

      Don’t know, what kind of fluorochrome, but it definitelly should be possiblle if there are DNA chips and similar stuff 😉
      Just join it to either end of the strain. If you wished to use amines anyway, you could join it to the carbonyl groups of basis, so than you will have affected binding to complementary strand 😉

    • #93386
      strasbarge
      Participant

      Thanks but actually I wanted to know how I could join an amine group to RNA basis precisely… I may not have been clear ^^

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