RNA precipitation after extraction: role of LiCl ?

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    • #10289
      faerstd
      Participant

      Hi,

      I have to write my protocol after a course in Molecular Biology, where in one lecture we extracted the RNA from the cells. After doing so, we precipitated the RNA with Ethanol and Lithium chloride for 3 hours.

      Now my question: what’s the role of LiCl in this precipitation? Wouldn’t the RNA be precipitated just with Ethanol?
      Can I say LiCl binds to other substances left after the extraction, like DNA ?

      have to understand why we used LiCl ..

      any ideas? please help.

      thanks in advance. faerstd

    • #86611
      MrMistery
      Participant
    • #86613
      faerstd
      Participant

      i’m so stupid.. i searched google indeed, but didn’t read that !!!! 😆

      so this licl specially gets large dehydrated RNA molecules make aggregates so that the precipitation works better

      thanks a lot.. 😳

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