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    • #18245
      ppm58
      Participant

      Hello

      I culture cells as 3D model (spheroid) embedded in agarose matrix. I am trying to recovery RNA from these culture to perform RT PCR but I meet several problem as RNA quantity (<30 ng/mL) and as RNA quality (260/280/230 ratio are bad).

      Have some ideas to obtain a fair RNA quality and quantity in these culture agarose conditions.

      Thank you for your help
      Pascal

    • #116032
      Fornita
      Participant

      This is a general question. For detail, how do you extract RNA? The most important thing is separating your cells and agarose, how did you do?

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