I got confusion about the function of Laemmli sample buffer….
Does Laemmli sample buffer lyse adherent cells for SDS-PAGE? or I need to lyse my cells first with a cell lysis solution and then dilute the cell lysate with laemmli buffer?
i think this laemmli buffer is just a type of cell lysis buffer. i looked at the recipe and it seems very similar to the sample buffer i use. I mean, BME, glycerol, SDS, bromphenol blue… looks like sample buffer to me