sample preparation for SDS-PAGE

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    • #13101
      roniadam
      Participant

      Hi

      I got confusion about the function of Laemmli sample buffer….
      Does Laemmli sample buffer lyse adherent cells for SDS-PAGE? or I need to lyse my cells first with a cell lysis solution and then dilute the cell lysate with laemmli buffer?

    • #99008
      JackBean
      Participant

      IMHO you have to lyse the cells first, but I’m not expert in PAGE

    • #99118
      MrMistery
      Participant

      i think this laemmli buffer is just a type of cell lysis buffer. i looked at the recipe and it seems very similar to the sample buffer i use. I mean, BME, glycerol, SDS, bromphenol blue… looks like sample buffer to me

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