Biology Forum › Molecular Biology › saving my minigel

last updated by blcr11 17 years, 3 months ago
2 voices
1 reply
  • Author
    Posts
    • August 18, 2007 at 9:29 pm #8100
      bluerose
      Participant

      Is there a way to save my minigel which I ran DNA onto already this morning, so that the bands don’t diffuse over the weekend? I was told that if I left it in the TAE buffer, my DNA will diffuse out after a few hours. Is there any way to prevent this long enough and save my gel? I need it for the future. Could I wrap it up and place it in the freezer? If so, at what temperature?

      Thanks

    • August 18, 2007 at 10:39 pm #75264
      blcr11
      Participant

      Not a great idea. About the best you can do is wrap it in saran wrap and put it in the cold, but there will still be some diffusion. If you’re extracting or transferring to a blot, you’re better off doing it right off rather than waiting til Monday. What, hot date or something?

  • Author
    Posts

You must be logged in to reply to this topic.

No related posts.

Members