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    • #9668
      Snuggles
      Participant

      I’m currently working on the rabies genome, and after the RT-PCR I sequence a region using both the forward and the reverse primers.

      How do I get BioEdit to "pair up" these sequences so that I get a double stranded sequence from the forward and reverse sequences?

    • #84212
      mith
      Participant

      we used sequencher, they had a "consensus" command.

    • #84223
      Snuggles
      Participant

      Ok, but let’s say that I only have access to BioEdit?

    • #84323
      MichaelXY
      Participant

      You might try emailing the author of Bioedit.
      thall@isisph.com

    • #84326
      Snuggles
      Participant

      I came right in the end, thanks anyways. 🙂

    • #104468
      kbowles
      Participant

      In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into "sequence" –> "nucleic acid" –> "reverse complement"

      I realize this is an old discussion but it might be helpful…

    • #106561
      merv
      Participant
      quote kbowles:

      In Bioedit line up the reverse sequence by editing it into the reverse complement form. It will now align with your fw sequence. Select the sequence you want, on the function tabs go into “sequence” –> “nucleic acid” –> “reverse complement”

      I realize this is an old discussion but it might be helpful…

      I think there is a similar command in Gene Jockey.
      Also, if you prefer submission, you can do so : http://www.bioinformatics.org/sms/rev_comp.html

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