Single PCR tube DNA Extraction Method

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    • #11289

      I need to make a DNA extraction Method that fits in a single 150ul PCR tube and can be PCRed following the completion of the method. So, here are my ideas and method and please comment and give suggestions.

      PCR Tube- Mixed inside PCR tube
      10ul of 1% SDS solution
      10ul of Protein K Kinase
      15ul of Nucleas free water.
      15 Beads from a Zippy Fecal Extraction kit, Then Vortexted at the highestl level for 30 minutes.

      PCR tube then Placed in a Boiling water bath for 15 miniutes then,
      35ul of TAQ mastermix
      1ul of fwr primer
      1ul of rev primer

      Then PCRed.

      Comments or suggestions?

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