I have a RAPD primer which produces a polymorphic band (of around 540 bp) for 5 species of a same genus. Now I want to convert to Species Specific Primer/SCAR. My expectation is one primer to differentiate all 5 species. It means I have to synthesis a single primer which will produce bands of different bp in electrophoresis gel. Is is possible? If so, please let me get the step by step procedure.