on the other hand after some thoughts, in a ordinary PCR cycle the annealing temp is about 50 deg and then rised to 72 for taq to begin. So I dont know but it might be a problem there. If taq was active at so much lower temps, it would already have replicated the whole gene before the temp hit 72, am I fishing out of the blue now?
what would you expect to happen when you use normal DNA polymerase in a PCR and you let the temperature rise to 95 degrees C for 15 sec to separate the DNA strands ? There lies the choice of using Taq-polymerase: it’s heat stable.