The best way to dissolve BSA - Biology Forum

last updated by

Hick 18 years, 7 months ago

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- May 16, 2006 at 7:35 am #4807
  Hick
  Participant
  Dear readers,
  how do you dissolve totally BSA?
  Are you shaking the fluidum?
  -> but it produces froam
  Do you use a Vortex
  -> it produces foam like shaking, even more
  Do you slightly shake and swing in a big bottle?
  Do you use a magnetic stirrer?
  -> one can say that the magnetic field has an influence on the polarized proteins, an argument, but…
  Do you use a magnetic stirrer with a temperature set? Which temperature do you use?
  -> the magnetic field and the temperature will change the proteins
  Thank`s for your informations and help,
  sincerly
  Hick
- May 17, 2006 at 9:14 am #48538
  liliane79
  Participant
  hey hick,
  i guess time and patience solves your problem… 8)
  take a look here:
  http://www.invitrogen.com/content/sfs/manuals/3118.pdf
  that may give you a good idea on how to best dissolve BSA. we do it that way and it works just fine.
  cheers,
  lili
- May 18, 2006 at 9:00 am #48595
  Hick
  Participant
  Hello,
  thank`s a lot for the PDF.
  Well time, that`s the stuff which is not in the cataloge of Sigma isn`t it??? 😀
  I wish you a nice day,
  see ya,
  greets HICK
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