[ti gon]

can somebody pls help me with my coursework. I ve 0nly got 2 days to do it. my teacher is very rigorous and i need to obtain a grade A to pass. the subject title is the effects of antibiotics on gram positive and gram negative bacteria. any sugestions???????? pleeeeez help ti gon. 🙁

[MrMistery]

All i know about this subject is that antibiotics stops the bacteria from dividing… Maybe Patrick knows some usefull sites for you…

[canalon]

I’d like to help but the question is a bit to vast to be answered here. There are books of more than 1000 of pages specifically treating the subject of which antibiotic is efficient against which kind of bacteria.

I found an interesting review on the subject here http://www.merck.com/mmhe/sec17/ch192/ch192a.html
and here:
http://pathmicro.med.sc.edu/mayer/antibiot.htm

It may help you start, but if you were more precise about what you need, or the limit of your subject I could give a more complete answer.

Patrick

[ti gon]

hiya yea sorry about the indistiction of my question. the bacterias i am using are ecoli and S.epidermis and the the antibiotics are ampicillin and Kanamycin. can you believe i have got to do a 100 pages on the subject??? its just absurd!! 👿 i practically begged for an extension and i got a 2 day extension. 😡 i really need to get this completed and i think with the help of my new friends patrick and Mr Mistry i hope i will have it done 😉 thanks alot pal XXXXXXXxxxx

[MrMistery]

Patrick is the one you should thank. I am just in the dark on this subject as you are.
Andrew

[ti gon]

YES. BUT YOU SUGGESTED I GET PATRICKS HELP. SO REALLY THANK YOU!

[MrMistery]

Lol… Welcome then 😀 😀 😀 😀

[canalon]

Sorry but I come back from a long week-end. I do really not see how you could do 100 pages on the subject with only 2 genus of bacteria and 2 antibiotics! Sure it is not 10 pages?

But what you could do:

– Determination of the minimal inhibitory concentration (MIC) of both antibiotics on both bacteria: Find a medium that is suitable for both (Mueller-Hinton is usually the one recommended, but I’m sure wether S. epidermis would grow on it) either agar plates or broth. Prepare different concentration of antibiotic and inoculate with approximately 10^6 bacteria per ml. Grow @37C for 16 to 20h and note which concentration inhibits growth. (in case of broth make triplicate experiment). Compare MIC for both species and discuss.
-Use Medline and the links I gave you and try to identify the targets of your antibiotics to compare the composition of the targets for both species.
– Have a lot of illustrations 😆

Back in the lab for further questions. Good Luck

Patrick

[ti gon]

NO PATRICK ITS NOT TEN ITS A HUNDRED ALRIGHT. BUT I HAVE FINALLY COMPLETED IT AND GUESS WOT????//// I GOT MY GRADE!!!!! WOOHOO!!!!! ALL THANKS TO YOU PAT. ANYWAY GO ANYWHERE NICE ON YOUR LONG WEEKEND????

[MrMistery]

Congratulations!!

[Poison]

Happy to hear that ti gon…

[canalon]

Good for you. I don’t know how you managed that, I wouldn’t have been able to…

Congratulations

[MrMistery]

You don’t remember what needing a grade is like, do you Patrick? 😀 😀 😀

[canalon]

No, I don’t rememeber sitting for any exam in the last 6 or 7 years….
And For this I trust my less than perfect memory 😆

No grades for me, I just need to convince people to publish my papers. And I am not sure it is easier.

Patrick

[b_d_41501]

For Gram – bacteria, the antibiotics work less efficiently due to the fact they have a lipopolysaccharide layer. The gram + bacteria lack this which allows more antibiotics to prove useful in treating these bacteria.

[MrMistery]

The main differenece between Gram= and Gram- is Gram+ have a thincker cellular wall and lack that lipoproteic membrane at the exterior that Gram- have. That membrane is similar in structure to the plasma membrane

[EmmVeePee]

So Gram – bacteria are more resistant to ALL antibiotics and not those that just target the cell wall (NAG and NAM)? Does the lipoproteic layer just protect it from the antibiotics?

[Author]

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