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    • #17161
      bravebeaker
      Participant

      Hi all,
      I have a question and I would like you to help me figure it out.
      Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this
      20 mM MES
      0.4 M mannitol
      20 mM KCl
      10 mM CaCl2
      0.1% BSA
      1.5% Cellulase and so on…
      I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume to work with? lets say I need 10 ml of this enzyme solution with the mentioned contents.

      Thank you very much for your help.

    • #113315
      JackBean
      Participant

      just put everything into one beaker

    • #113352
      bravebeaker
      Participant

      Well Jack bean… I was waiting with anticipation for your reply actually but not kind of that reply.. very elaborative 😛 but thanks anyways 😀

    • #113353
      JackBean
      Participant

      just weight all chemicals, put them into one beaker and let dissolve. That’s how you make buffers.

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