I have a question and I would like you to help me figure it out.
Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this
20 mM MES
0.4 M mannitol
20 mM KCl
10 mM CaCl2
1.5% Cellulase and so on…
I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume to work with? lets say I need 10 ml of this enzyme solution with the mentioned contents.