Thioredoxin

Viewing 2 reply threads
  • Author
    Posts
    • #11904
      piefke
      Participant

      Hi!

      I need thioredoxin from plant or cyanobacteria for an enzyme assay. Does anybody work with such TRX? Where did you get it from? I only found TRX from E. coli…

    • #93242
      JackBean
      Participant

      Find some article about characterization of such protein and try to contact author. Maybe will s/he send you vector in some bacteria

      (What are you about to do? )

    • #93329
      MrMistery
      Participant

      for an enzyme assay? your best bet is probably to do what hjackbean suggested. then clone the gene into a plasmid with some sort of purification tag (His Tag, or even better Tap tag), and then purify it careful so your protein doesn’t denature in the process.

      As for getting it, there are a probably a million papers dealing with thioredoxin, so finding someone to give you some DNA won’t be such a big deal. If you prefer, you can also get it from addgene – bacterial as well as human variants of the gene are available.
      http://www.addgene.org/pgvec1?identifie … pl&x=0&y=0

Viewing 2 reply threads
  • You must be logged in to reply to this topic.