To whom working with DNA sequencing

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    • #9184
      roniadam
      Participant

      1. Would a primer extension reaction in DNA sequencing work using an RNA polymerase? any one can explain this point please?
      2. When the products of a DNA sequencing reaction are separated by electrophoresis, why is it normally only possible to read about 500 to 800 bases, yet the sequencing reaction continues for thousands of bases?

    • #82343
      canalon
      Participant

      1- look up initiation of the reaction for both enzymes. besides think about RNA stability.
      2- think about the termination mechanism and the relation between probability of synthesis of a fragment of increasing length and the intensity of the signal.

    • #82388
      roniadam
      Participant

      Still cannot find the answers

    • #82395
      canalon
      Participant

      read this, and notably paragraph 3.1:

      http://en.wikipedia.org/wiki/Rna_Polymerase

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