Top-down and bottom-up technology

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    • #14403

      Hi there,

      I was wondering if anybody is able to explain top-down and bottom-up technology in terms of DNA sequencing? I have no clue how it works and I have minimal notes on it!

      Thanks 🙂


    • #103268

      I would guess it’s like this:
      top-down – you first sort your chromosomes, then make from them smaller and smaller pieces (but you somehow know the order) and these pieces you sequence
      bottom-up – you just break all your DNA, sequence (preferentially on Next-Gen sequencing machines) and from these small pieces you assemble the whole genome.

      But I’m just guessing, I have never heared it in relation to DNA sequencing

    • #103270

      Thanks 🙂 I’m not sure if that’s how it goes, but I don’t know myself either so I can’t really say! I’m trying to look in my text book for it but no luck so far 😕

      Seren x

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