Viewing 1 reply thread
  • Author
    • #17036

      I am going to be using TOPO cloning for the first time. I am using the Invitrogen p-ET-102 Directional cloning kit.

      My question is the following:

      Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I’m not sure if that mean’s I’ll have my product chopped along the way to create that blunt-end.

      Anyone ever use this kit before? I had to add "CACC" to the 5′ of my forward primers as a recognition site but I don’t know if it uses my entire recombinant protein or not.


    • #112978

      That depends only on your primers, to which position are they designed.

Viewing 1 reply thread
  • You must be logged in to reply to this topic.