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    • #17036
      coppcw08
      Participant

      I am going to be using TOPO cloning for the first time. I am using the Invitrogen p-ET-102 Directional cloning kit.

      My question is the following:

      Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I’m not sure if that mean’s I’ll have my product chopped along the way to create that blunt-end.

      Anyone ever use this kit before? I had to add "CACC" to the 5′ of my forward primers as a recognition site but I don’t know if it uses my entire recombinant protein or not.

      Thanks!

    • #112978
      JackBean
      Participant

      That depends only on your primers, to which position are they designed.

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