Biology Forum › Community › General Discussion › Tween

last updated by Dr.Stein 18 years, 1 month ago
5 voices
9 replies
  • Author
    Posts
    • November 29, 2006 at 1:52 am #6440
      Dr.Stein
      Participant

      Hello, I am Dr.Stein 😉

      I need a reference, or based to your own experience, of Tween concentration used to emulsify 70% ethanolic extract into distilled water. I have Tween 20 (10% stock). This extract will be administered orally as much as 0.5 mL to mouse (30 gram weight) for 34-67 days. Thank you 🙂

      Please reply me ASAP! 😆

    • November 29, 2006 at 9:05 am #61368
      Dr.Stein
      Participant

      None? 😥 😥

    • November 29, 2006 at 9:14 am #61369
      Dustfinger
      Participant

      What is Tween ?
      I don’t understand your question…..

    • November 30, 2006 at 2:02 pm #61474
      jnkdna
      Participant

      me neither. though i doubt i cud help u. i can always try!

    • December 1, 2006 at 1:32 am #61514
      Dr.Stein
      Participant

      Tween is a harmless detergent for laboratory use with a pH balance about 6.8-7.2. It is applied to rinse something like chemicals, antibodies, and another liquids out from our tubes, plates, wells, or another places. It also is applied to emulsify lipids or any alcohol-based stuff like ethanolic extracts into aqueous environment, so it will dissolved completely. In our body system, we have bile to do this emulsion 😉

    • December 1, 2006 at 9:19 am #61556
      Dustfinger
      Participant

      Then I’m sorry I don’t know the answer……

    • December 1, 2006 at 2:42 pm #61575
      Dr.Stein
      Participant

      It’s alright 🙂 Luckily, my 70% ethanolic extract is completely dissolved on the distilled water, so that I didn’t need tween to emulsify 😉 However, I am still expecting any asnwers to anticipate if in the future I have extract that is not easily dissolved 😀

    • December 1, 2006 at 4:22 pm #61582
      canalon
      Participant

      I used it at 0.02% but it was more as a wettig agent than an emulsifying agent. I might suggest that you try to find something that is hard to dissolve in water but does in ethanol (oil for example should do at low concentration) and make serial dilutions of tween and see which is the lowest concentration that works.

      Since you work with a worst case scenario (oil is completely insoluble in water) it should work with anything else. don’t you think?

    • December 13, 2006 at 1:19 am #62628
      boy_interrupted
      Participant

      How about using Sodium Taurocholate? Or is it too strong?

    • December 13, 2006 at 10:57 am #62670
      Dr.Stein
      Participant

      I never use it, have no experience yet. I am afraid my bioactive substance will react or at least change into something inactive with that 🙄 Tween is neutral 🙂

  • Author
    Posts

You must be logged in to reply to this topic.

No related posts.

Members