Vector - Biology Forum

last updated by

citroenboom 12 years, 2 months ago

3 voices

2 replies

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- August 12, 2012 at 5:10 pm #16746
  siddharthsameer
  Participant
  hello all
  I would like to ask,is that i already of the used up my vector, and I plated the vector on one of the lb plate which i used as a negative control at the time of doing ligation,,So i would be trying to make the preculture from that and then the main culture and would do the miniprep to get my vector..I can easily know the concentration through UV but how do I get the mass of my vector…My vector is pPICZalphaA ….
  since i am doing all this thing for the first time, so kindly help me…..
  regards
- August 22, 2012 at 7:34 pm #112144
  triton
  Participant
  I dont get it. What do you mean by MASS??? If the total amount i.e. weight, so simply multiply your concentration by the total volume…
- September 3, 2012 at 11:36 am #112228
  citroenboom
  Participant
  Maybe check the marker? Then you will find the amount of basepairs.
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