I need to know what plasmid construct lacks the PolyA signal. I need this vector because I want to use an insert which contain the polyA signal so is there any vector I can purchase without the polyA signal.
Here is a vector without a SV40 poly A signal, but you did not mention any other requirements.
Check invitrogen for other possibilities.
http://products.invitrogen.com/ivgn/en/ … tKey=88101