i have a quistion about restriction enzymes.what is it how can it be used and what is the procces of recombinant? ❓ 😕
Restriction enzymes like endonuclease, ECO R1 cut DNA at particular sequences like GAATTC ..
Then this DNA fragment can be inserted to a plasmid which can be transfered to a vector( bacteria ) to multiply it and to produce a gene library.
usually they cleave DNA at so called palindromes. Recall that besides cohesive ends, blunt ends can also be generated, which are more difficult to ligate in a plasmid.