June 5, 2005 at 8:36 am #1077
what is the full length transcripts?
and what are the difference between the full length transcipts and mRNA, I sure that they are not the same things? Because in the paper said that almost mRNA sequences in GenBank canot be represent full-length transcripts. and the full-length transcripts are, on average, 45 bp longer than mRNA.
and why is the full length transcipts longer than mRNA ?
Are the full length cDNA and full length transcripts the same or not ?
Thank you very much in advance
June 5, 2005 at 10:12 am #23803quote iboon:
I’ll try to explain. Gene carries genetic information for 1)protein 2)tRNA 3)rRNA.
Gene consists of exones(coding regions-which will be translated into polypeptide chain)and intrones(non-coding regions which must be cut-cause you don’t translate them). First during transcription complementary RNA is formed(full length transcipt or primary transcript) -it consists of egzones+intrones so whole gene is trancribed. Then by special mechanism intrones are cut so you get mRNA(consist only of exones). During translation mRNA is translated to polypeptide chain of aminoacids.
Hope that helps! 😀
June 5, 2005 at 12:23 pm #23806quote :
that mean full length transcipt = exon + intron + 5′ UTR + 3′ UTR
mature mRNA = exon + 5′ UTR + 3′ UTR
In the paper said that “the full-length transcripts are, on average, 45 bp longer than mRNA.(Consensus Promoter Identification in the Human Genome Utilizing Expressed Gene Markers and Gene Modeling :Rongxiang Liu, and David J. States, In part of analysis of genes for which a functionally cloned mRNA is avialable) ”
if u say like that the average of length should be longer than 45 bp cause the intron in thefull length transcipt is longer than 45 bp. i think
i dont sure i understand correctlly or not pls suggest me
Thank you for your reply
June 5, 2005 at 4:58 pm #23830
mRNA not of exon but exones and primary transcript not of exon and introne but exones and intrones
like this gene exone1-introne1-exone2-introne2….. primary transcript exone1-introne1-exone2-introne2... mRNA…exone1-exone2
Gene can be much larger than unit that codes for protein(as u can conclude)Introns vary while exones are quite conserved(cause selection removes carriers of severe mutations in exones). Very long genes are the result of very long introns! In some techiniques you compare lengths of of alleles. That could be a sign of genetical relation. Could, but not definitely cause same lengh doesn’t mean same nucleotide order. But if u compare more polymorphic regions and get covering in size between 2 individuals, the possibility that they are related is higher(again same size doesn’t mean same nucleotide order).
June 6, 2005 at 6:05 am #23861
almost mRNA sequences in GenBank canot be represent full-length transcripts. and the full-length transcripts are, on average, 45 bp longer than mRNA.
do u agree with ?
June 6, 2005 at 1:15 pm #23885quote iboon:
Never heard that information. And cause it says on average if you are going to put it in your work I would suggest to skip it. It is more important to know differences between primary transcript and mRNA.That’s all. And if u mean on genetic libraries well it’s not so hard to constate size of gene so than you’ll now which vector to use(plasmid, virus, BAC,YAC,….)
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